What is the Elisa test for?

What is the Elisa test for?

ELISA can be used for several diseases in addition to HIV, being a technique that allows the detection of specific antibodies in the blood. In this type of test, the presence of the virus is not directly investigated, but rather the existence of antibodies against it.

How is the immunofluorescence test performed?

Immunofluorescence is defined as a technique that enables the visualization of antigens in tissues or cell suspensions, through the use of specific antibodies, labeled with fluorochrome, capable of absorbing ultra-violet (UV) light, emitting it at a certain length of time. wave, allowing its…

What is the immunofluorescence test?

It is an examination performed on unfixed biopsy material (generally skin and kidney) to demonstrate the presence of immunoglobulins. This exam helps in the diagnosis and classification of lesions such as glomerulopathies and some skin diseases. The Direct Immunofluorescence technique is based on an antigen-antibody reaction.

What is the main difference between the Elisa and Immunofluorescence methods?

It is based on checking reactions between antigens and antibodies, through the immunoenzymatic test. The immunofluorescence method uses specific antibodies marked by a substance called fluorochrome, a fluorescent dye that allows the virus to be identified through observation via a microscope.

What does immunoenzimatic mean?

The immunoenzymatic reaction is the basis for many tests used to identify antigens or antibodies. The system involves antibodies conjugated to enzymes.

What does the word antigen mean?

The term antigen or immunogen means any molecular species of biological origin isolated or consisting of a cell, virus, biological or synthetic liquid that when introduced into a vertebrate organism (called host or receptor) is capable of producing an immune reaction.

What does the word Enzymeimmunoassay mean?

The enzyme immunoassay test (ELISA) to detect the presence of antibodies against the HIV virus, for example, may present false positive results, in some cases, due to the interference of some factors, such as patients with rheumatoid arthritis, autoimmune diseases, acute viral infection , immunological disease of…

What is the diagnostic window?

Diagnostic Window : it is a broader concept than the immunological or serological window . The diagnostic window period is the time elapsed between infection and the appearance or detection of an infection marker, be it viral RNA, proviral DNA, p24 antigen or antibody.

What is the immunological window for syphilis?

In the case of syphilis , the immunological window varies according to the stage of the disease, however, in most cases, it is already possible to detect antibodies against Treponema pallidum, the causative agent of syphilis , around 3 weeks after infection.

What does the word suggestive mean?

1. Which contains suggestion; that suggests (e.g.: suggestive metaphor; suggestive texts ). 2. That attracts or fascinates (e.g. suggestive clothing ).

When the non-reactive Beta result means what?

What should I do if my test was non-reactive (negative)? A non-reactive (negative) test means that your body does not have antibodies against HIV at the time of testing.

How to read the Beta-hCG result?

Here’s how to interpret a beta hCG test :

  1. Up to 5 mIU/mL: negative beta hCG . Indicates that there is no pregnancy;
  2. From 5 to 25 mIU/mL: beta hCG inconclusive. …
  3. Above 25 mIU/mL: positive beta hCG .

How to read pregnancy test results?

Beta hcg values ​​and possible results

  1. BhCG below 5 mIU/ml: negative or undetectable result, there is no indication of pregnancy .
  2. BhCG between 5 and 25 mIU/ml: undefined result. It is recommended to repeat the exam after three days to be sure about the pregnancy.
  3. BhCG above 25 mIU/ml: positive result, indicates pregnancy .

What is the hCG level to be pregnant?

hCG levels during pregnancy

3 weeks (UPM) 5 – 50 mIU/ml
13-16 weeks (UPM) 13,300 – 254,000 mIU/ml
17-24 weeks (UPM) 4,060 – 165,400 mIU/ml
25-40 weeks (UPM) 3,640 – 117,000 mIU/ml
Non-pregnant women

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